Plasma mutation
technology
technology
ARTP-IIS
Product overviewProduct parameters
Successful cases
Product overview
Compared with the traditional low-pressure gas
discharge plasma source, Atmospheric Room Temperature Plasma (ARTP)
has the characteristics of low plasma jet temperature, uniform discharge and
high concentration of chemically active particles. Based on ARTP technology,
Our company, together with the Department of chemical engineering and the
Department of Engineering Physics of Tsinghua University, has jointly developed
the first special instrument for mutation and breeding of microorganisms by
means of plasma — ARTP Mutagenesis Breeding Machine.
The instrument has high mutation rate, compact structure, simple operation,
high safety and fast mutation speed. A large mutation library can be obtained
by one mutation operation (within a few minutes), which greatly improves the
intensity of strain mutation and the capacity of mutation library; ARTP
technology combined with high-throughput screening technology can realize rapid
and efficient evolutionary breeding of organisms.
Compared with the traditional low-pressure gas
discharge plasma source, Atmospheric Room Temperature Plasma (ARTP)
has the characteristics of low plasma jet temperature, uniform discharge and
high concentration of chemically active particles. Based on ARTP technology,
Our company, together with the Department of chemical engineering and the
Department of Engineering Physics of Tsinghua University, has jointly developed
the first special instrument for mutation and breeding of microorganisms by
means of plasma — ARTP Mutagenesis Breeding Machine.
The instrument has high mutation rate, compact structure, simple operation,
high safety and fast mutation speed. A large mutation library can be obtained
by one mutation operation (within a few minutes), which greatly improves the
intensity of strain mutation and the capacity of mutation library; ARTP
technology combined with high-throughput screening technology can realize rapid
and efficient evolutionary breeding of organisms.
Product parameters
Successful cases
Case 1： Using ARTP to Improve the Activity of Glutaminase Produced by Streptothrix Streptoverticillium Mobaraense
MTGase is a transferase that catalyzes acyl transfer reaction. It is used to change the structure and functional characteristics of proteins. It is widely used in food, textile, biopharmaceutical and other fields. The core link is to obtain MTGase high-yield strains. Due to the low plasma temperature, it will not cause thermal damage to the strains, and the high concentration of active particles can produce obvious mutagenic effect, so it is suitable for biological breeding.
Using ARTP technology to mutate Streptomyces spores, the mutation rate was 42.8%, the positive mutation rate was 20.6%, and the enzyme activity of high-yield mutant G2-1 reached 2.73u/ml, which was 82% higher than that of the original strain
Typical
colony characteristics after ARTP mutation
(G1-G14 is the representative of typical colonies with different
morphology from the original strain; G15 is the same morphology as the original
strain)
Case 2： Construction of Spirulina Mutant Library by ARTP and Breeding of High Polysaccharide Producing Mutant
Microalgae can fix carbon dioxide through photosynthesis to produce renewable biomass. Therefore, as an alternative energy for chemical fuels, the development of microalgae bioenergy has received extensive attention in the world. At present, microalgae with fast growth, high polysaccharide content, good salt tolerance, good flocculation and high chlorophyll content are urgently needed.
Using ARTP to mutate spirulina, mutant 3-b2 showed strong flocculation, and the flocculation degree reached more than 90%, while the flocculation degree of original Spirulina was about 65%.
Morphology of Spirulina and its three mutants in shake flask
Case 1： Using ARTP to Improve the Activity of Glutaminase Produced by Streptothrix Streptoverticillium Mobaraense
MTGase is a transferase that catalyzes acyl transfer reaction. It is used to change the structure and functional characteristics of proteins. It is widely used in food, textile, biopharmaceutical and other fields. The core link is to obtain MTGase high-yield strains. Due to the low plasma temperature, it will not cause thermal damage to the strains, and the high concentration of active particles can produce obvious mutagenic effect, so it is suitable for biological breeding.
Using ARTP technology to mutate Streptomyces spores, the mutation rate was 42.8%, the positive mutation rate was 20.6%, and the enzyme activity of high-yield mutant G2-1 reached 2.73u/ml, which was 82% higher than that of the original strain
Typical
colony characteristics after ARTP mutation
(G1-G14 is the representative of typical colonies with different
morphology from the original strain; G15 is the same morphology as the original
strain)
Case 2： Construction of Spirulina Mutant Library by ARTP and Breeding of High Polysaccharide Producing Mutant
Microalgae can fix carbon dioxide through photosynthesis to produce renewable biomass. Therefore, as an alternative energy for chemical fuels, the development of microalgae bioenergy has received extensive attention in the world. At present, microalgae with fast growth, high polysaccharide content, good salt tolerance, good flocculation and high chlorophyll content are urgently needed.
Using ARTP to mutate spirulina, mutant 3-b2 showed strong flocculation, and the flocculation degree reached more than 90%, while the flocculation degree of original Spirulina was about 65%.
Morphology of Spirulina and its three mutants in shake flask
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